2024（令和6年）年度「科学的リテラシーのトレーニング：上級編（第6期）」として、Journal Clubを行なっています。徹底的理解と批判的読解力、プレゼン力、質疑応答力、自らの研究への応用力を鍛えています。Pre-JCでは、課題論文のコアデータを紹介し概要を把握して議論し、最新の知見を総覧します（浅読みのトレーニング）。JCでは、Pre-JCから選択した論文を、徹底的に読み込み、議論します（深読みのトレーニング）。また、東京大学大学院医学系研究科・疋田温彦先生研究室との合同JCで、研究室間交流を行います。
2024年度は45報の論文について議論しました。

20250311
東京大学大学院医学系研究科・疋田温彦先生研究室との合同JC
発表者：孝口　里侑（北海道大学　口腔診断内科学教室）
Zhai, Y. et al.
Differential bone and vessel type formation at superior and dura periosteum during cranial bone defect repair.
Bone Res 13, 8 (2025).
https://doi.org:10.1038/s41413-024-00379-9
発表者：疋田　温彦　先生　（東京大学）
Bixel, M. G. et al.
Angiogenesis is uncoupled from osteogenesis during calvarial bone regeneration.
Nat Commun 15, 4575 (2024).
https://doi.org:10.1038/s41467-024-48579-5

20250304 Pre Journal Club
発表者：西本　慎之介
Nakano-Kobayashi, A. et al.
Astrocyte-targeting therapy rescues cognitive impairment caused by neuroinflammation via the Nrf2 pathway.
Proc Natl Acad Sci U S A 120, e2303809120 (2023).
https://doi.org:10.1073/pnas.2303809120
Bixel, M. G. et al.
Angiogenesis is uncoupled from osteogenesis during calvarial bone regeneration.
Nat Commun 15, 4575 (2024).
https://doi.org:10.1038/s41467-024-48579-5
Flury, A. et al.
A neurodegenerative cellular stress response linked to dark microglia and toxic lipid secretion.
Neuron 113, 554-571 e514 (2025).
https://doi.org:10.1016/j.neuron.2024.11.018

20250225 Journal Club
発表者：西浦　まい
CRISPR-Cas9 screens reveal regulators of ageing in neural stem cells. Nature 634, 1150-1159 (2024).
https://doi.org:10.1038/s41586-024-07972-2

20250218 Pre Journal Club
発表者：孝口　里侑
Liu, D. et al.
Transcriptome and metabolome analysis of osteoblasts identifies disrupted purine metabolism and parathyroid hormone associated pathway induced by P. gingivalis infection.
Bone 193, 117401 (2025).
https://doi.org:10.1016/j.bone.2025.117401
Zhai, Y. et al.
Differential bone and vessel type formation at superior and dura periosteum during cranial bone defect repair.
Bone Res 13, 8 (2025).
https://doi.org:10.1038/s41413-024-00379-9
Furuhashi, K. et al.
Bone marrow niches orchestrate stem-cell hierarchy and immune tolerance.
Nature 638, 206-215 (2025).
https://doi.org:10.1038/s41586-024-08352-6
20250204 Journal Club

20250204 Journal Club
発表者：上野　楓美
Mapping RANKL- and OPG-expressing cells in bone tissue: the bone surface cells as activators of osteoclastogenesis and promoters of the denosumab rebound effect.
Bone Res 12, 62 (2024).
https://doi.org:10.1038/s41413-024-00362-4

20250128 Pre Journal Club
発表者：西浦　まい
He, L. et al.
Inhibition of NFAT5-Dependent Astrocyte Swelling Alleviates Neuropathic Pain. Adv Sci (Weinh) 11, e2302916 (2024).
https://doi.org:10.1002/advs.202302916
Ruetz, T. J. et al.
CRISPR-Cas9 screens reveal regulators of ageing in neural stem cells. Nature 634, 1150-1159 (2024).
https://doi.org:10.1038/s41586-024-07972-2
Zhang, Q. Y. et al.
Regulation of enzymatic lipid peroxidation in osteoblasts protects against postmenopausal osteoporosis. Nat Commun 16, 758 (2025).
https://doi.org:10.1038/s41467-025-55929-4

20250121 Journal Club
発表者：渡辺　陽久
Nakamura, K. et al. The periosteum provides a stromal defence against cancer invasion into the bone.
Nature 634, 474-481 (2024).
https://doi.org:10.1038/s41586-024-07822-1

20250107 Pre Journal Club
発表者：上野　楓美
Courbon, G. et al.
FGF23 directly inhibits osteoprogenitor differentiation in Dmp1-knockout mice.
JCI Insight 8 (2023).
https://doi.org:10.1172/jci.insight.156850
Yu, T. et al.
Colon-targeted engineered postbiotics nanoparticles alleviate osteoporosis through the gut-bone axis.
Nat Commun 15, 10893 (2024).
https://doi.org:10.1038/s41467-024-55263-1
El-Masri, B. M. et al.
Mapping RANKL- and OPG-expressing cells in bone tissue: the bone surface cells as activators of osteoclastogenesis and promoters of the denosumab rebound effect.
Bone Res 12, 62 (2024).
https://doi.org:10.1038/s41413-024-00362-4

20241217
東京大学大学院医学系研究科・疋田温彦先生研究室との合同JC

発表者：辻 直紀　先生　（東京大学）
Mohanakrishnan, V. et al.
Specialized post-arterial capillaries facilitate adult bone remodelling.
Nat Cell Biol 26, 2020-2034 (2024).
https://doi.org:10.1038/s41556-024-01545-1
発表者：西本　慎之介　（北海道大学　歯科矯正教室）
M2 macrophages secrete glutamate-containing extracellular vesicles to alleviate osteoporosis by reshaping osteoclast precursor fate.
Mol Ther 32, 1158-1177 (2024).
https://doi.org:10.1016/j.ymthe.2024.02.005

20241210 Pre Journal Club
発表者：渡辺　陽久
Zou, N. Y. et al.
Age-related secretion of grancalcin by macrophages induces skeletal stem/progenitor cell senescence during fracture healing.
Bone Res 12, 6 (2024).
https://doi.org:10.1038/s41413-023-00309-1
Choudhury, D. et al.
Proline restores mitochondrial function and reverses aging hallmarks in senescent cells.
Cell Rep 43, 113738 (2024). https://doi.org:10.1016/j.celrep.2024.113738
Nakamura, K. et al. The periosteum provides a stromal defence against cancer invasion into the bone.
Nature 634, 474-481 (2024). https://doi.org:10.1038/s41586-024-07822-1

20241203 Pre Journal Club
発表者：西本　慎之介
Xia, C. et al.
beta-catenin inhibition disrupts the homeostasis of osteogenic/adipogenic differentiation leading to the development of glucocorticoid-induced osteonecrosis of the femoral head.
Elife 12 (2024).
https://doi.org:10.7554/eLife.92469
Huang, X. et al.
M2 macrophages secrete glutamate-containing extracellular vesicles to alleviate osteoporosis by reshaping osteoclast precursor fate.
Mol Ther 32, 1158-1177 (2024).
https://doi.org:10.1016/j.ymthe.2024.02.005
Ruan, B. et al.
DNMT aberration-incurred GPX4 suppression prompts osteoblast ferroptosis and osteoporosis.
Bone Res 12, 68 (2024).
https://doi.org:10.1038/s41413-024-00365-1

20241126 Journal Club
発表者：高田健太郎 （理学院自然史科学専攻　小林快次教授研究室）
Narita, Y. & Kuratani, S.
Evolution of the vertebral formulae in mammals: a perspective on developmental constraints.
J Exp Zool B Mol Dev Evol 304, 91-106 (2005).
https://doi.org:10.1002/jez.b.21029

20241119 Journal Club
発表者：孝口　里侑
Shu, Y. et al.
Inhibition of inflammatory osteoclasts accelerates callus remodeling in osteoporotic fractures by enhancing CGRP(+)TrkA(+) signaling. Cell Death Differ (2024).
https://doi.org:10.1038/s41418-024-01368-5

20241112 Journal Club
発表者：西浦　まい
Babey, M. E. et al.
A maternal brain hormone that builds bone. Nature 632, 357-365 (2024).
https://doi.org:10.1038/s41586-024-07634-3
Alvarez, Z. et al.

20241015 Pre-Journal Club
発表者：孝口　里侑
Park, K. M. et al.
Preventive effect of teriparatide on medication-related osteonecrosis of the jaw in rats. Sci Rep 13, 15518 (2023).
https://doi.org:10.1038/s41598-023-42607-y
Hsu, C. C. et al.
Daily supplement of sesame oil prevents postmenopausal osteoporosis via maintaining serum estrogen and aromatase levels in rats. Sci Rep 14, 321 (2024).
https://doi.org:10.1038/s41598-023-50453-1
Shu, Y. et al.
Inhibition of inflammatory osteoclasts accelerates callus remodeling in osteoporotic fractures by enhancing CGRP(+)TrkA(+) signaling. Cell Death Differ (2024).
https://doi.org:10.1038/s41418-024-01368-5

20240924 Journal Club
発表者：西本　慎之介
Ju, Y. H. et al.
Tonic excitation by astrocytic GABA causes neuropathic pain by augmenting neuronal activity and glucose metabolism.
Exp Mol Med 56, 1193-1205 (2024).
https://doi.org:10.1038/s12276-024-01232-z

20240917 Pre-Journal Club
発表者：西浦　まい
Babey, M. E. et al.
A maternal brain hormone that builds bone. Nature 632, 357-365 (2024).
https://doi.org:10.1038/s41586-024-07634-3
Alvarez, Z. et al.
Artificial extracellular matrix scaffolds of mobile molecules enhance maturation of human stem cell-derived neurons. Cell Stem Cell 30, 219-238 e214 (2023).
https://doi.org:10.1016/j.stem.2022.12.010
Hu, Z. et al.
CNTF-STAT3-IL-6 Axis Mediates Neuroinflammatory Cascade across Schwann Cell-Neuron-Microglia. Cell Rep 31, 107657 (2020).
https://doi.org:10.1016/j.celrep.2020.107657

20240910
東京大学大学院医学系研究科・疋田温彦先生研究室との合同JC

発表者：上野　楓美　（北海道大学　歯科矯正学教室）
Jahn, D. et al.
Increased beta(2)-adrenergic signaling promotes fracture healing through callus neovascularization in mice.
Sci Transl Med 16, eadk9129 (2024).
https://doi.org:10.1126/scitranslmed.adk9129
発表者：辻 直紀　先生　（東京大学）
Stewart, K. S. et al.
Stem cells tightly regulate dead cell clearance to maintain tissue fitness. Nature 633, 407-416 (2024).
https://doi.org:10.1038/s41586-024-07855-6

20240716 Pre-Journal Club
発表者：西本　慎之介
Ju, Y. H. et al.
Tonic excitation by astrocytic GABA causes neuropathic pain by augmenting neuronal activity and glucose metabolism.
Exp Mol Med 56, 1193-1205 (2024).
https://doi.org:10.1038/s12276-024-01232-z
Huang, J. et al.
beta-Receptor blocker enhances the anabolic effect of PTH after osteoporotic fracture.
Bone Res 12, 18 (2024).
https://doi.org:10.1038/s41413-024-00321-z
Pei, F. et al.
FGF signaling modulates mechanotransduction/WNT signaling in progenitors during tooth root development.
Bone Res 12, 37 (2024).
https://doi.org:10.1038/s41413-024-00345-5

20240716 Pre-Journal Club
発表者：上野　楓美
Hansen, M. S. et al.
Transcriptional reprogramming during human osteoclast differentiation identifies regulators of osteoclast activity.
Bone Res 12, 5 (2024).
https://doi.org:10.1038/s41413-023-00312-6
Ding, Y. et al.
Early protection against bone stress injuries by mobilization of endogenous targeted bone remodeling.
iScience 26, 107605 (2023).
https://doi.org:10.1016/j.isci.2023.107605
Jahn, D. et al.
Increased beta(2)-adrenergic signaling promotes fracture healing through callus neovascularization in mice.
Sci Transl Med 16, eadk9129 (2024).
https://doi.org:10.1126/scitranslmed.adk9129

20240709 Journal Club
発表者：発表者：高田健太郎 （理学院自然史科学専攻　小林快次教授研究室）1
Marsicano, C. A. et al.
Giant stem tetrapod was apex predator in Gondwanan late Palaeozoic ice age.
Nature (2024).
https://doi.org:10.1038/s41586-024-07572-0

20240611 Journal Club
発表者：孝口　里侑
Luo, Z. et al.
Neutrophil hitchhiking for drug delivery to the bone marrow.
Nat Nanotechnol 18, 647-656 (2023).
https://doi.org:10.1038/s41565-023-01374-7

20240521
東京大学大学院医学系研究科・疋田温彦先生研究室との合同JC

発表者：西浦まい　（北海道大学　小児・障害者歯科学教室）
Donovan, L. J. et al.
Repopulated spinal cord microglia exhibit a unique transcriptome and contribute to pain resolution.
Cell Rep 43, 113683 (2024).
https://doi.org:10.1016/j.celrep.2024.113683
発表者：Ahmed Elsherbini先生　（東京大学）
Sun, J. et al.
A vertebral skeletal stem cell lineage driving metastasis.
Nature 621, 602-609 (2023).
https://doi.org:10.1038/s41586-023-06519-1

20240514 Pre-Journal Club
発表者：孝口　里侑
Atkinson, E. G. et al.
Conditional Loss of Nmp4 in Mesenchymal Stem Progenitor Cells Enhances PTH-Induced Bone Formation.
J Bone Miner Res 38, 70-85 (2023).
https://doi.org:10.1002/jbmr.4732
Huang, J. et al.
beta-Receptor blocker enhances the anabolic effect of PTH after osteoporotic fracture.
Bone Res 12, 18 (2024).
https://doi.org:10.1038/s41413-024-00321-z
Luo, Z. et al.
Neutrophil hitchhiking for drug delivery to the bone marrow.
Nat Nanotechnol 18, 647-656 (2023).
https://doi.org:10.1038/s41565-023-01374-7

20240507 Pre-Journal Club
発表者：渡辺　陽久
Guo, T. et al.
Vascular architecture regulates mesenchymal stromal cell heterogeneity via P53-PDGF signaling in the mouse incisor.
Cell Stem Cell (2024).
https://doi.org:10.1016/j.stem.2024.04.011
Dourson, A. J. et al.
Macrophage memories of early-life injury drive neonatal nociceptive priming.
Cell Rep 43, 114129 (2024). https://doi.org:10.1016/j.celrep.2024.114129
Wang, D. et al.
Cell membrane vesicles derived from hBMSCs and hUVECs enhance bone regeneration.
Bone Res 12, 23 (2024).
https://doi.org:10.1038/s41413-024-00325-9

20240423 Journal Club
発表者：西本　慎之介
Sun, S. et al.
CHIT1-positive microglia drive motor neuron ageing in the primate spinal cord.
Nature 624, 611-620 (2023).
https://doi.org:10.1038/s41586-023-06783-1

202400409 Journal Club
発表者：上野　楓美
Ahmad, M. et al.
Inhibition of Cdk5 increases osteoblast differentiation and bone mass and improves fracture healing. Bone Res 10, 33 (2022).
https://doi.org:10.1038/s41413-022-00195-z

20240416 Pre-Journal Club
発表者：西浦　まい
Liao, P. et al.
Osteocyte mitochondria regulate angiogenesis of transcortical vessels.
Nat Commun 15, 2529 (2024).
https://doi.org:10.1038/s41467-024-46095-0
Shao, X. et al.
Rescuing SERCA2 pump deficiency improves bone mechano-responsiveness in type 2 diabetes by shaping osteocyte calcium dynamics.
Nat Commun 15, 890 (2024).
https://doi.org:10.1038/s41467-024-45023-6
Donovan, L. J. et al.
Repopulated spinal cord microglia exhibit a unique transcriptome and contribute to pain resolution.
Cell Rep 43, 113683 (2024).
https://doi.org:10.1016/j.celrep.2024.113683