（概要）
2023（令和5年）年度「科学的リテラシーのトレーニング：上級編（第5期）」として、Journal Clubを行なっています。徹底的理解と批判的読解力、プレゼン力、質疑応答力、自らの研究への応用力を鍛えています。Pre-JCでは、課題論文のコアデータを紹介し概要を把握して議論し、最新の知見を総覧します（浅読みのトレーニング）。JCでは、Pre-JCから選択した論文を、徹底的に読み込み、議論します（深読みのトレーニング）。また、東京大学大学院医学系研究科・疋田温彦先生研究室との合同JCで、研究室間交流を行います。

2023年度は41報の論文について議論しました。

20240319
東京大学大学院医学系研究科・疋田温彦先生研究室との合同JC
発表者：小野紗也加　先生　先生（東京大学）
Frenz-Wiessner, S. et al.
Generation of complex bone marrow organoids from human induced pluripotent stem cells.
Nat Methods (2024).
https://doi.org:10.1038/s41592-024-02172-2
発表者：渡辺陽久　（北海道大学　口腔診断内科学教室）
Ling, Z. et al.
Parathyroid hormone treatment partially reverses endplate remodeling and attenuates low back pain in animal models of spine degeneration.
Sci Transl Med 15, eadg8982 (2023).
https://doi.org:10.1126/scitranslmed.adg8982

20240312 Pre-Journal Club
発表者：西本慎之介
Liu, Y. et al.
Targeted knockdown of PGAM5 in synovial macrophages efficiently alleviates osteoarthritis.
Bone Res 12, 15 (2024).
https://doi.org:10.1038/s41413-024-00318-8
Rohatgi, N. et al.
BAP1 promotes osteoclast function by metabolic reprogramming.
Nat Commun 14, 5923 (2023).
https://doi.org:10.1038/s41467-023-41629-4
Sun, S. et al.
CHIT1-positive microglia drive motor neuron ageing in the primate spinal cord.
Nature 624, 611-620 (2023).
https://doi.org:10.1038/s41586-023-06783-1

202400305 Pre-Journal Club
発表者：上野　楓美
Wolfel, E. M. et al.
Reduced Bone Mass and Increased Osteocyte Tartrate-Resistant Acid Phosphatase (TRAP) Activity, But Not Low Mineralized Matrix Around Osteocyte Lacunae, Are Restored After Recovery From Exogenous Hyperthyroidism in Male Mice.
J Bone Miner Res 38, 131-143 (2023).
https://doi.org:10.1002/jbmr.4736
Ahmad, M. et al.
Inhibition of Cdk5 increases osteoblast differentiation and bone mass and improves fracture healing.
Bone Res 10, 33 (2022).
https://doi.org:10.1038/s41413-022-00195-z
Chen, G. et al.
Circular RNA circStag1 promotes bone regeneration by interacting with HuR.
Bone Res 10, 32 (2022).
https://doi.org:10.1038/s41413-022-00208-x

20240220 Journal Club
発表者：上野　楓美
Nie, T. et al.
Estradiol increases cortical and trabecular bone accrual and bone strength in an adolescent male-to-female mouse model of gender-affirming hormone therapy.
Bone Res 12, 1 (2024).
https://doi.org:10.1038/s41413-023-00308-2

20240213 Journal Club
発表者：西本 慎之介
Schafer, S. T. et al.
An in vivo neuroimmune organoid model to study human microglia phenotypes.
Cell 186, 2111-2126 e2120 (2023).
https://doi.org:10.1016/j.cell.2023.04.022

20240206 Journal Club
発表者：西浦　まい
Guo, Q. et al.
Unloading-Induced Skeletal Interoception Alters Hypothalamic Signaling to Promote Bone Loss and Fat Metabolism.
Adv Sci (Weinh) 10, e2305042 (2023).
https://doi.org:10.1002/advs.202305042

20240131 Pre-Journal Club
発表者：渡辺　陽久
Nie, T. et al.
Estradiol increases cortical and trabecular bone accrual and bone strength in an adolescent male-to-female mouse model of gender-affirming hormone therapy.
Bone Res 12, 1 (2024).
https://doi.org:10.1038/s41413-023-00308-2
Collins, J. M. et al.
YAP and TAZ couple osteoblast precursor mobilization to angiogenesis and mechanoregulation in murine bone development.
Dev Cell 59, 211-227 e215 (2024).
https://doi.org:10.1016/j.devcel.2023.11.029
Sunadome, K. et al.
Directionality of developing skeletal muscles is set by mechanical forces.
Nat Commun 14, 3060 (2023).
https://doi.org:10.1038/s41467-023-38647-7

20240123 Pre-Journal Club
発表者： 星（沼端）　麻里絵
Schafer, S. T. et al.
An in vivo neuroimmune organoid model to study human microglia phenotypes.
Cell 186, 2111-2126 e2120 (2023).
https://doi.org:10.1016/j.cell.2023.04.022
Su, G. et al.
Annexin A5 derived from matrix vesicles protects against osteoporotic bone loss via mineralization.
Bone Res 11, 60 (2023).
https://doi.org:10.1038/s41413-023-00290-9
Sun, Q. et al.
Parathyroid hormone attenuates osteoarthritis pain by remodeling subchondral bone in mice.
Elife 10 (2021).
https://doi.org:10.7554/eLife.66532

20240116 Pre-Journal Club
発表者：西浦　まい
Obeidat, A. M. et al.
Piezo2 expressing nociceptors mediate mechanical sensitization in experimental osteoarthritis.
Nat Commun 14, 2479 (2023).
https://doi.org:10.1038/s41467-023-38241-x
Tanaka, T. et al.
Dermal macrophages set pain sensitivity by modulating the amount of tissue NGF through an SNX25-Nrf2 pathway.
Nat Immunol 24, 439-451 (2023).
https://doi.org:10.1038/s41590-022-01418-5
Guo, Q. et al.
Unloading-Induced Skeletal Interoception Alters Hypothalamic Signaling to Promote Bone Loss and Fat Metabolism.
Adv Sci (Weinh) 10, e2305042 (2023).
https://doi.org:10.1002/advs.202305042

20231212　東京大学大学院医学系研究科・疋田温彦先生研究室との合同JC
発表者：西浦　まい　（北海道大学　小児・障がい者歯科学教室）
Xu, J. et al.
NGF-p75 signaling coordinates skeletal cell migration during bone repair.
Sci Adv 8, eabl5716 (2022).
https://doi.org:10.1126/sciadv.abl5716
発表者：王　智煒　先生（東京大学）
Jaber, Y. et al.
Langerhans cells shape postnatal oral homeostasis in a mechanical-force-dependent but microbiota and IL17-independent manner.
Nat Commun 14, 5628 (2023).
https://doi.org:10.1038/s41467-023-41409-0

20231121 Pre-Journal Club
発表者：西浦　まい
Xu, J. et al.
NGF-p75 signaling coordinates skeletal cell migration during bone repair.
Sci Adv 8, eabl5716 (2022).
https://doi.org:10.1126/sciadv.abl5716
Du, J. et al.
BRD9-mediated chromatin remodeling suppresses osteoclastogenesis through negative feedback mechanism.
Nat Commun 14, 1413 (2023).
https://doi.org:10.1038/s41467-023-37116-5
Zhao, X. et al.
Yap and Taz promote osteogenesis and prevent chondrogenesis in neural crest cells in vitro and in vivo.
Sci Signal 15, eabn9009 (2022).
https://doi.org:10.1126/scisignal.abn9009

20231031
発表者：高田健太郎 （理学院自然史科学専攻　小林快次教授研究室）
Ciancio, M. R., Krmpotic, C. M., Scarano, A. C. & Epele, M. B.
Internal Morphology of Osteoderms of Extinct Armadillos and Its Relationship with Environmental Conditions.
J Mamm Evol 26, 71-83 (2019).
https://doi.org:10.1007/s10914-017-9404-y

20231010 Journal Club
発表者：星（沼端）　麻里絵
Ono, T. et al.
Simultaneous augmentation of muscle and bone by locomomimetism through calcium-PGC-1alpha signaling.
Bone Res 10, 52 (2022).
https://doi.org:10.1038/s41413-022-00225-w

20230919東京大学大学院医学系研究科・疋田温彦先生研究室との合同JC
発表者：辻　直紀　先生（東京大学）
Matsushita, Y. et al. Bone marrow endosteal stem cells dictate active osteogenesis and aggressive tumorigenesis.
Nat Commun 14, 2383 (2023).
https://doi.org:10.1038/s41467-023-38034-2
発表者：渡辺陽久先生（北海道大学　口腔診断内科学教室・薬理学教室）
Biswas, L. et al. Lymphatic vessels in bone support regeneration after injury.
Cell 186, 382-397 e324 (2023).
https://doi.org:10.1016/j.cell.2022.12.031

20230912 Pre-Journal Club
発表者：渡辺　陽久
Biswas, L. et al. Lymphatic vessels in bone support regeneration after injury.
Cell 186, 382-397 e324 (2023).
https://doi.org:10.1016/j.cell.2022.12.031
Diegel, C. R. et al.
Inhibiting WNT secretion reduces high bone mass caused by Sost loss-of-function or gain-of-function mutations in Lrp5.
Bone Res 11, 47 (2023).
https://doi.org:10.1038/s41413-023-00278-5
Kim, P. et al.
Mast4 determines the cell fate of MSCs for bone and cartilage development.
Nat Commun 13, 3960 (2022).
https://doi.org:10.1038/s41467-022-31697-3

20230718東京大学大学院医学系研究科・疋田温彦先生研究室との合同JC
発表者：星（沼端） 麻里絵　（北海道大学　歯科矯正学教室・薬理学教室）
Arostegui, M., Scott, R. W. & Underhill, T. M.
Hic1 identifies a specialized mesenchymal progenitor population in the embryonic limb responsible for bone superstructure formation.
Cell Rep 42, 112325 (2023).
https://doi.org:10.1016/j.celrep.2023.112325
発表者：疋田先生（東大）
Suh, J. et al.
Mitochondrial fragmentation and donut formation enhance mitochondrial secretion to promote osteogenesis.
Cell Metab 35, 345-360 e347 (2023).
https://doi.org:10.1016/j.cmet.2023.01.003

20230711 Journal Club
発表者：西浦　まい
Shi, S. et al.
Glia maturation factor beta deficiency protects against diabetic osteoporosis by suppressing osteoclast hyperactivity.
Exp Mol Med 55, 898-909 (2023).
https://doi.org:10.1038/s12276-023-00980-8

20230704 Pre-Journal Club
発表者：星（沼端）　麻里絵
Arostegui, M., Scott, R. W. & Underhill, T. M.
Hic1 identifies a specialized mesenchymal progenitor population in the embryonic limb responsible for bone superstructure formation.
Cell Rep 42, 112325 (2023).
https://doi.org:10.1016/j.celrep.2023.112325
Geoghegan, I. P., McNamara, L. M. & Hoey, D. A.
Estrogen withdrawal alters cytoskeletal and primary ciliary dynamics resulting in increased Hedgehog and osteoclastogenic paracrine signalling in osteocytes. Sci Rep 11, 9272 (2021).
https://doi.org:10.1038/s41598-021-88633-6
Ono, T. et al.
Simultaneous augmentation of muscle and bone by locomomimetism through calcium-PGC-1alpha signaling.
Bone Res 10, 52 (2022).
https://doi.org:10.1038/s41413-022-00225-w

20230606 Pre Journal Club
発表者：西浦　まい
Hu, Y. et al.
Dual roles of hexokinase 2 in shaping microglial function by gating glycolytic flux and mitochondrial activity.
Nat Metab 4, 1756-1774 (2022).
https://doi.org:10.1038/s42255-022-00707-5
Shi, S. et al.
Glia maturation factor beta deficiency protects against diabetic osteoporosis by suppressing osteoclast hyperactivity.
Exp Mol Med 55, 898-909 (2023).
https://doi.org:10.1038/s12276-023-00980-8
Zhai, X. et al.
Molecular insights into the distinct signaling duration for the peptide-induced PTH1R activation.
Nat Commun 13, 6276 (2022).
https://doi.org:10.1038/s41467-022-34009-x

20230530 Journal Club
発表者：星（沼端）麻里絵
Wu, Y. et al.
Reduced osteoclast-derived apoptotic bodies in bone marrow characterizes the pathological progression of osteoporosis.
Cell Death Discov 9, 135 (2023).
https://doi.org:10.1038/s41420-023-01434-w

20230516 Journal Club
発表者：渡辺　陽久
Dzamukova, M. et al.
Mechanical forces couple bone matrix mineralization with inhibition of angiogenesis to limit adolescent bone growth.
Nat Commun 13, 3059 (2022).
https://doi.org:10.1038/s41467-022-30618-8

20230509 Pre-Journal Club
発表者：星（沼端）麻里絵
Hayashi, Y. et al.
MDS cells impair osteolineage differentiation of MSCs via extracellular vesicles to suppress normal hematopoiesis.
Cell Rep 39, 110805 (2022).
https://doi.org:10.1016/j.celrep.2022.110805
Ponte, F. et al.
Mmp13 deletion in mesenchymal cells increases bone mass and may attenuate the cortical bone loss caused by estrogen deficiency.
Sci Rep 12, 10257 (2022).
https://doi.org:10.1038/s41598-022-14470-w
Wu, Y. et al.
Reduced osteoclast-derived apoptotic bodies in bone marrow characterizes the pathological progression of osteoporosis.
Cell Death Discov 9, 135 (2023).
https://doi.org:10.1038/s41420-023-01434-w

20230425 Journal Club
発表者：西浦　まい
Sato, T. et al.
Comparable Initial Engagement of Intracellular Signaling Pathways by Parathyroid Hormone Receptor Ligands Teriparatide, Abaloparatide, and Long-Acting PTH.
JBMR Plus 5, e10441 (2021).
https://doi.org:10.1002/jbm4.10441

20230418 Pre-Journal Club
発表者：渡辺　陽久
Qin, Q. et al. Neuron-to-vessel signaling is a required feature of aberrant stem cell commitment after soft tissue trauma.
Bone Res 10, 43 (2022).
https://doi.org:10.1038/s41413-022-00216-x
Dzamukova, M. et al.
Mechanical forces couple bone matrix mineralization with inhibition of angiogenesis to limit adolescent bone growth.
Nat Commun 13, 3059 (2022).
https://doi.org:10.1038/s41467-022-30618-8
Zhang, Z. et al.
Programmable integrin and N-cadherin adhesive interactions modulate mechanosensing of mesenchymal stem cells by cofilin phosphorylation.
Nat Commun 13, 6854 (2022).
https://doi.org:10.1038/s41467-022-34424-0
Kase, Y. et al.
The original strain of SARS-CoV-2, the Delta variant, and the Omicron variant infect microglia efficiently, in contrast to their inability to infect neurons: Analysis using 2D and 3D cultures. Exp Neurol 363, 114379 (2023). https://doi.org:10.1016/j.expneurol.2023.114379